Establishment of a cell line from larvae of Culex pipiens fatigans and its susceptibility to infection with some group C arboviruses.
نویسندگان
چکیده
Os autores relatam o estabelecimento de uma nova linhagem de células obtidas a partir de mosquitos Culex pipiens fatigans colonizados em laboratório. Empregando esta nova linhagem de células foi realizado um estudo comparativo de sua susceptibilidade a cinco arbovírus do Grupo C: Apeu.Marituba, Murutucu, Oribocae Itaqui. Todos os cinco tipos de arbovírus foram capazes de infectar células, demonstrando um aumento no título de 0,5 a 2,0 unidades logarítmicas. A infectividade foi demonstrada por sub-inoculaçao em camundongos recém-natos e cultura de células He La e não pelo efeito citopatogênico das células de mosquito. Foi realizada também uma comparação entre a susceptibilidade desta nova linhagem e um estudo similar empregando células de Aedes albopictus.
منابع مشابه
LOCAL PRODUCTION OF PRIMARY POWDER OF BACILLUS THURINGIENSIS SEROTYPE H-14 IN IRAN AND DETERMINATION OF ITS INSECTICIDAL PROPERTIES AGAINST CULEX PIPIENS AND ANOPHELES STEPHENSI
Three media formulated from molasses and cornsteep liquor and another media based on the basal medium formulation of IPS-82 were assessed for the growth and production of insecticidal properties of B. thuringiensis H-14 .• Bacterial powders prepared from the broth cultures were assayed against the larvae of Culex pipiens and Anopheles stephensi. A standard powder of IPS-82 was included in ...
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متن کامل
Characterization of phospholipase B of Culex pipiens fatigans.
Phospholipase B has been found in the mosquito Culex pipiens fatigans, and some of its properties have been studied. The enzyme had a high optimum temperature (45 degrees C) and broad alkaline pH optimum (8-9). It was inactive toward diacylphospholipids, and hydrolyzed lysolecithin at a higher rate than lysophosphatidyl ethanolamine. The enzyme was heat labile, but lysolecithin protected it aga...
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Background & Objective: Epidemic of dangerous diseases caused by Culex pipiens’ bites prompted us to focus on these groups of insects. Today, the most successful biological insecticides are produced by the bacteria of the genus Bacillus, which in the production phase of spores, makes crystalline endotoxin protein. This protein can be activated upon entering the insect’s intestine in alkali...
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Introduction & Objective: The aim of the study was to identify larval habitats and new species and determine the exact distribution of Culicidae in Hamadan Province. Materials & Methods: This descriptive, cross-sectional study was conducted in six regions of Hamadan Province during 2010. Sampling method was carried out by standard Dipping method. Specimens were mounted by lactophenol solutio...
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ورودعنوان ژورنال:
- Revista latinoamericana de microbiologia
دوره 21 4 شماره
صفحات -
تاریخ انتشار 1979